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human β ngf  (R&D Systems)


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    R&D Systems human β ngf
    Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+ngf/Recombinant+Human+beta-NGF+Protein/pm41999764-275-45-47
    Average 95 stars, based on 111 article reviews
    human β ngf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Recombinant:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Article Title: TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors
    Article Snippet: .. The cervical trachea was exposed, and 50 μl (at designated concentrations) of human recombinant BDNF, human recombinant GDNF (BioSource, Invitrogen), human recombinant NGF (R&D Systems, Minneapolis, MN), or PBS (vehicle) was injected into the site along the upper tracheal as described above for DiI injections. ..

    Article Title: TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors.
    Article Snippet: .. The cervical trachea was exposed, and 50 l (at designated concentrations) of human recombinant BDNF, human recombinant GDNF (BioSource, Invitrogen), human recombinant NGF (R&D Systems, Minneapolis, MN), or PBS (vehicle) was injected into the site along the upper tracheal as described above for DiI injections. ..


    Article Title: Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways
    Article Snippet: .. Human recombinant NGF and VEGF, neutralizing antibodies against NGF, VEGF and the corresponding isotype control antibodies were purchased from R&D Systems. .. Growth factor-reduced Matrigel was from BD Biosciences.

    Article Title: Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells.
    Article Snippet: Neurotrophins (NTs) are expressed during tooth development.. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells.. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells.

    Saline:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Centrifugation:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    SYBR Green Assay:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Polymerase Chain Reaction:

    Article Title: Effects of the nerve growth factor and its carrier protein on the inflammatory response from human monocytes.
    Article Snippet: .. Bovine serum albumin were purchased from Sigma (Merck, Darmstadt, Germany); “DuoSet” enzyme linked immunosorbent assay (ELISA) kits and human recombinant NGF were supplied by R&D systems (Biotechne, Minneapolis, USA); CD14 magnetic microbeads kit was supplied by Miltenyi Biotech (Bergish, Germany); ethylene diamine tetra acetic acid (EDTA) was purchased as “0.5M pH 8.0 Ultrapure EDTA” from Invitrogen (Waltham, USA); phosphate buffered saline (PBS), RPMI 1640 medium, L-Glutamine, and Penicillin-Streptomycin were purchased from Gibco (Life Technologies, USA); fetal bovine serum (FBS) “Hyclone” came from Fischer scientific (USA); Ficoll centrifugation tubes are from eurobio scientific (France); RT-PCR kit “High-capacity cDNA Achieve Kit,” the “Power SYBR Green PCR MasterMix,” the thermocycler “QuantStudioTM 7 Flex System” bought from Applied Biosystems (Invitrogen, Waltham, USA). ..

    Injection:

    Article Title: TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors
    Article Snippet: .. The cervical trachea was exposed, and 50 μl (at designated concentrations) of human recombinant BDNF, human recombinant GDNF (BioSource, Invitrogen), human recombinant NGF (R&D Systems, Minneapolis, MN), or PBS (vehicle) was injected into the site along the upper tracheal as described above for DiI injections. ..

    Article Title: TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors.
    Article Snippet: .. The cervical trachea was exposed, and 50 l (at designated concentrations) of human recombinant BDNF, human recombinant GDNF (BioSource, Invitrogen), human recombinant NGF (R&D Systems, Minneapolis, MN), or PBS (vehicle) was injected into the site along the upper tracheal as described above for DiI injections. ..

    Incubation:


    Control:

    Article Title: Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways
    Article Snippet: .. Human recombinant NGF and VEGF, neutralizing antibodies against NGF, VEGF and the corresponding isotype control antibodies were purchased from R&D Systems. .. Growth factor-reduced Matrigel was from BD Biosciences.



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    Image Search Results


    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis

    doi: 10.1038/s41467-026-70000-6

    Figure Lengend Snippet: Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Article Snippet: The mixture was placed in an incubator at 37 °C with 5% CO 2 for 30 min, followed by stimulation with recombinant human NGF (R&D Systems, 256-GF) at a final concentration 0.1 μg/mL (or PBS as negative control) and the resulting mixtures were further incubated for 20 min. Next, anti-CD123-APC (Miltenyi Biotec, 130-115-265) and anti-CD203c-PE (Miltenyi Biotec, 130-092-343) antibodies were added and continued to incubate for another 15 minutes.

    Techniques: RNA Expression, Control, Expressing, RNA Sequencing